Deformable Tissue Aligner

Perform non-rigid B-Spline image registration to correct local tissue deformations across multiplexed RAM-FISH hybridization rounds.

Ground truth structural anchor (e.g., Round 1 DAPI / Housekeeping gene).

The shifted reference channel from the subsequent imaging round.

The RNA-FISH signal from Round 2 that needs to be warped.

⚠️ Beta Testing Notice: Cloud resources are currently running on a limited free tier (512MB RAM). Please downsample or crop large files (e.g., to ~1200px) before uploading to avoid memory overloads.

Awaiting Image Sequence

Upload both tracking references and your target channel to begin alignment.