RAM-FISH

A gallery of multiplexer, geneplexer, and rembot images. The RAM-FISH (Rapid Amplified Multiplexed-FISH) automation platform is a custom-engineered robotics suite designed to scale high-resolution spatial mRNA profiling. Operating in a continuous, closed-loop cycle, the system utilizes a precision Multiplexer for automated cyclic probe labeling and fluidic delivery. Following high-resolution confocal imaging, samples are processed by the RemBot, a dedicated signal-removal robot that efficiently clears fluorescence to prepare the tissue for subsequent multiplexing rounds. By integrating custom microcontrollers and modular fluidics, this platform significantly streamlines complex spatial biology workflows.