RGB-HCR Probe Pool Designer

Pool Name: Gene 1 Sequence: Gene 2 Sequence: Gene 3 Sequence: Gene 4 Sequence: Gene 5 Sequence: Gene 6 Sequence: GC Content Threshold (%): 30% 40% 50% 60% 70% Gap between Probe Sets (bp): Design Probes Download Excel

Cite: Banerjee, T. D., Raine, J., Mathuru, A. S., Chen, K. H., and Monteiro, A. (2024). Spatial mRNA profiling using Rapid Amplified Multiplexed-FISH (RAM-FISH). bioRxiv. Link

References: Choi, H. M. T., Schwarzkopf, M., Fornace, M. E., Acharya, A., Artavanis, G., Stegmaier, J., Cunha, A. and Pierce, N. A. (2018). Third-generation in situ hybridization chain reaction : multiplexed , quantitative , sensitive , versatile , robust. Development. Link